Figure 5. Disulfiram protected against Alcohol-Induced bone loss via ALDH1A1. (A) Sections of tibias were stained with H&E and TRAP. (B) Data of quantitative analyses of histomorphometric bone parameters of the bone volume to tissue volume (BV/TV), number of TRAP-positive OCs, and percentage of the OC surface to bone surface (Oc.S/BS, %) (n = 6). (C) Immunohistochemical staining of ALDH1A1 expression in the liver. (D) Immunohistochemical staining of ALDH1A1 expression in the tibia. (E) qPCR was conducted to measure the relative levels of gene expression, normalized to those of β-actin, in each group of mice (n = 6). Bar graphs are presented as the mean ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001 compared to the respective controls.