Research Paper Volume 11, Issue 19 pp 8120—8138

The interaction between STAT3 and nAChRα1 interferes with nicotine-induced atherosclerosis via Akt/mTOR signaling cascade

Figure 4. A novel interaction between nAChRα1 and STAT3 occurs in MOVAS and RAW264.7 cells. (A) Coimmunoprecipitation of nAChRα1 with STAT3 in MOVAS cells. (B) The direct binding of nAChRα1 and STAT3 in RAW264.7 cells. (C) Coimmunoprecipitation of nAChRα1 with STAT3 in 293T cells. (D) The colocalization of nAChRα1 and STAT3 in MOVAS cells, as determined by confocal microscopy. Magnification, 630×; bars, 50 μm. IP, immunoprecipitation, IB, immunoblot. Each experiment was performed three times.