Research Paper Volume 11, Issue 19 pp 8139—8155

Hypermethylated long noncoding RNA MEG3 promotes the progression of gastric cancer

Figure 2. The expression and methylation status of target factors in gastric cancer and gastric cancer cell lines. (A) LncRNA MEG3 was found to be expressed at low levels in thirty GC samples compared to normal tissues. **P<0.01, compared with 30 matched normal tissues. (B) LncRNA MEG3 was also found to be downregulated in BGC-823, SGC-7910, MGC-803 and AGS GC cell lines compared to the GES 1 normal cell line. *P<0.05, **P<0.01, compared with the GES 1 normal cell line. (C) MSP results showed that lncRNA MEG3 was hypermethylated in tumor tissues compared with normal tissues. **P<0.01, compared with 30 matched normal tissues. (D) Hypermethylation of lncRNA MEG3 was also detected in BGC-823, SGC-7910, MGC-803 and AGS GC cell lines compared to the GES 1 normal cell line. *P<0.05, **P<0.01, compared with the GES 1 normal cell line. (E) MiR-181a-5p was upregulated in tumor tissues compared with their corresponding normal tissues. **P<0.01, compared with 30 matched normal tissues. (F) QRT-PCR results showed higher expression of miR-181a-5p in BGC-823, SGC-7910, MGC-803 and AGS GC cell lines compared to the GES 1 normal cell line. *P<0.05, **P<0.01, compared with the GES 1 normal cell line. (G) QRT-PCR results showed lower expression of ATP4B in tumor tissues compared to normal tissues. **P<0.01, compared with 30 matched normal tissues. (H) QRT-PCR results showed higher expression of ATP4B in BGC-823, SGC-7910, MGC-803 and AGS GC cell lines compared to the GES 1 normal cell line. *P<0.05, **P<0.01, compared with the GES 1 normal cell line.