Research Paper Volume 11, Issue 19 pp 8156—8168

Knockdown of CREB3 activates endoplasmic reticulum stress and induces apoptosis in glioblastoma

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Figure 4. The downregulation of CREB3 induced apoptosis in U251MG cells. (A) CREB3 levels were detected by qRT-PCR in U251MG cells transfected with the NC, CREB3-shRNA1 or CREB3-shRNA1. (B, C) CREB3 levels were measured by Western blotting in U251MG cells transfected with the NC, CREB3-shRNA1 or CREB3-shRNA2. β-actin was used as a loading control. (D, E) U251MG cells were transfected with CREB3-shRNA2 for 72 h. Apoptosis was detected with Annexin V and PI double staining, and the percentage of apoptotic cells was calculated. (F) BAX and active caspase 3 levels were detected by Western blotting in U251MG cells transfected with CREB3-shRNA2 for 72 h. (G) The relative levels of BAX and active caspase 3 were quantified and normalized to β-actin levels. *P<0.05, **P<0.01 compared with the NC group.