Research Paper Volume 11, Issue 19 pp 8183—8203

CircRNA Cdr1as functions as a competitive endogenous RNA to promote hepatocellular carcinoma progression

Figure 1. Functions of circRNA Cdr1as in HCC cell lines. (A) Expression level of circRNA Cdr1as remained higher in HCC cell lines (SMMC-7721, Bel-7402, HepG2, Hep3B, Huh-7, HB611) than human normal liver cell line HL-7702 detected by qRT-PCR. (B) qRT-PCR detection of the relative expression of circRNA Cdr1as in paired HCC tumor and paired para-carcinoma tissues (n=42). (C) The sequence of circRNA Cdr1as in circBase (upper panel) was consistent with the result of Sanger sequencing (lower panel). (D) CircRNA Cdr1as was resistant to RNaseR digestion in HCC cell lines. (E) CCK8 assay showed proliferation of HepG2 and SMMC-7721 cells transfected with circRNA Cdr1as shRNA or overexpression vector. (F, G) EdU assay showed proliferation of HepG2 and SMMC-7721 cells transfected with circRNA Cdr1as shRNA or overexpression vector. (H, I) Transwell migration assay showed that down-regulation of circRNA Cdr1as inhibited the migration of HepG2 cells, and overexpression of circRNA Cdr1as promoted the migration of SMMC-7721 cells. Photographs were taken under an optical microscope with a magnification of 200×. Results were presented as mean ± SD. *P<0.05, **P<0.01, ***P<0.001. All of the experiments were performed in triplicate.