Research Paper Volume 11, Issue 19 pp 8183—8203

Figure 5. miR-1270/AFP regulatory loop plays an important role in cell biological Behavior. (A) Western blot analysis of AFP level in HepG2 cells transfected with miR-1270 inhibitor (with or without AFP shRNA). GAPDH was used as a control. (B) Western blot analysis of AFP level in SMMC-7721 cells transfected with miR-1270 mimics (with or without AFP overexpression vector). (C) EdU assay showed proliferation of HepG2 cells. (D) EdU assay showed proliferation of SMMC-7721 cells. (E) Transwell assay showed migration of HepG2 cells. (F) Transwell assay showed migration of SMMC-7721 cells. Results were presented as mean ± SD. *P<0.05, **P<0.01, ***P<0.001. versus control group. All of the experiments were performed in triplicate.