Research Paper Volume 11, Issue 19 pp 8239—8253

The long non-coding RNA, urothelial carcinoma associated 1, promotes cell growth, invasion, migration, and chemo-resistance in glioma through Wnt/β-catenin signaling pathway

Figure 2. Knock-down of UCA1 inhibited cell growth and increased apoptotic rate in glioma cells. (A and B) CKK-8 assay. The cell growth in (A) U87 and (B) SHG139 cells at 0, 24, 48 and 72 h after UCA1 siRNAs (siUCA1(a) and siUCA1(b)) or scrambled siRNA transfection. (C and D) Flow cytometry. The cell apoptotic rate in (C) U87 and (D) SHG139 cells after UCA1 siRNAs (siUCA1(a) and siUCA1(b)) or scrambled siRNA transfection. (E and F): Western blotting. The protein expression of Bcl-2, active caspase 3, and active caspase 9 in (E) U87 and (F) SHG139 cells after UCA1 siRNAs (siUCA1(a) and siUCA1(b)) or scrambled siRNA transfection. 1, scrambled siRNA; 2, siUCA1(a); 3, siUCA1(b). All the experiments were performed in triplicates. Significant differences compared to the control group were expressed as *P<0.05 and **P<0.01.