Research Paper Volume 11, Issue 19 pp 8239—8253

The long non-coding RNA, urothelial carcinoma associated 1, promotes cell growth, invasion, migration, and chemo-resistance in glioma through Wnt/β-catenin signaling pathway

Figure 4. Overexpression of UCA1 promoted cell proliferation, cell invasion and migration in glioma cells. The relative expression of UCA1 in (A) U87 and (B) SHG139 cells after pcDNA3.1-UCA1 (UCA1) or pcDNA3.1 (negative control) transfection was determined by qRT-PCR assay. The cell proliferative potential in (C) U87 and (D) SHG139 cells after pcDNA3.1-UCA1 (UCA1) or pcDNA3.1 (negative control) transfection was determined by CCK-8 assay. The cell invasive potential in (E) U87 and (F) SHG139 cells after pcDNA3.1-UCA1 (UCA1) or pcDNA3.1 (negative control) transfection was determined by cell invasion assay. The cell migratory potential in (G) U87 and (H) SHG139 cells after pcDNA3.1-UCA1 (UCA1) or pcDNA3.1 (negative control) transfection by wound healing assay. All the experiments were performed in triplicates. Significant differences compared to the control group were expressed as *P<0.05, **P<0.01 and ***P<0.001.