Research Paper Volume 11, Issue 19 pp 8254—8269

Soluble klotho regulates the function of salivary glands by activating KLF4 pathways

Figure 6. Functional restoration of PSGCs by soluble klotho and KLF4 expression. (A) Detection of the CD90 and CD44 protein levels in soluble klotho- or KLF4-expressing PSGCs. Cells were transfected with soluble klotho or KLF4 expression plasmids. At 48 h after transfection, total protein was prepared and subjected to immunoblotting. (B) Expression of KLF4, aquaporin 5, ZO-1, and amylase in PSGCs after transfection with soluble klotho or KLF4 expression plasmids. (C) Immunofluorescence staining of aquaporin 5, ZO-1, and amylase in PSGCs cultured on 3D Matrigel at day 5.