Figure 3. SA-T cells accumulate in lacrimal glands of aged mice, particularly in female mice. (A and B) Naïve (CD44loCD62Lhi) and effector memory (CD44hiCD62Llo) expression in CD4+ T cells (A), or PD-1+CD153+ expression in effector memory CD4+ T cells (B) in the lacrimal glands of young and aged mice. Results are representative of those from each group of mice. (C and D) Proportions (N=5) (C) and numbers adjusted by the lacrimal gland (LG) weight (N=4) (D) of PD-1+CD153+ cells gated on effector memory CD4+ T cells in the LG of young and aged mice. (E) CD153 mRNA expression levels in the LG of young and aged female mice, and of female mice fed a normal diet (ND) or high-fat diet (HFD) for 8 weeks (N=4-5). Values are presented as means ± SEM. *p<0.05 and **p<0.01 (an unpaired Student’s t-test).