Figure 3. Effects of mogroside V on spindle formation and chromosome alignment in oocytes during in vitro ageing. After in vitro maturation for 44 h, oocytes that extruded the first polar body were continuously cultured in vitro with or without MV for 24 h. (A) Representative images of spindle morphologies and chromosome alignment in fresh, aged and aged+MV oocytes. (B) The percentage of aberrant spindle formation in fresh, aged and aged+MV oocytes. (C) The percentage of misaligned chromosomes in fresh, aged and aged+MV oocytes. The data are presented as the mean ± SEM of at least three independent experiments. MV, mogroside V; Scale bar = 7.5 μm. ** P<0.01, *** P< 0.001.