Figure 4. MiR-134-5p targets MBTD1, which is regulated by lncRNA TTN-AS1. (A) The website predicted that MBTD1 was the target gene of miR-134-5p. (B, C) The luciferase assay confirmed that miR-134-5p targets MBTD1. (D–H) The effects of down-regulating miR-134-5p on MBTD1 mRNA and protein expression levels are shown. (I–K) The effects of upregulation of lncRNA TTN-AS1 on MBTD1 protein expression are shown. (L–N) The effects of knockdown of lncRNA TTN-AS1 and miR-134-5p on MBTD1 protein expression are shown. *P < 0.05 vs. mimic-NC, inhibitor-NC or sh-NC + inhibitor-NC group; #P < 0.05 vs. sh-TTN-AS1 + inhibitor-NC.