Figure 6. Rapamycin inhibits H2S production and expression of CBS and CGL in human cells. (A and B) H2S production was monitored in the presence or absence of Cys, PLP and rapamycin as indicated in 293T (A) or HeLa (B) cell. (C and D) Relative mRNA levels of CBS (C) or CGL (D) in HeLa cell treated with rapamycin for indicated times. Expression levels of β-actin mRNA were used as internal controls. (E). Western-blotting detection of CBS and CGL protein in HeLa cell treated with rapamycin for indicated times (Left). LC3 was also monitored to verify that the autophagy was induced by rapamycin. Quantification of CBS and CGL protein levels was based on Western blots and normalized to respective β-actin levels (Middle and Right). (* p<0.05).