Research Paper Volume 11, Issue 19 pp 8623—8641

TRIM59 loss in M2 macrophages promotes melanoma migration and invasion by upregulating MMP-9 and Madcam1

Figure 6. Knockdown of MMP-9 or Madcam1 inhibits melanoma growth in vivo. (A) Representative image of B16-F10 tumors from three independent experiments. WT or TRIM59-CKO mice were inoculated subcutaneously with B16-F10 cells transfected with control shRNA, MMP-9-shRNA, or Madcam1-shRNA. Tumor volumes were measured twice a week, and tumor weights estimated on sacrifice at day 28. n = 5 mice per group. Data are represented as mean ± SD. *p<0.05, **p<0.01, compared to tumors generated by shRNA control B16 cells in TRIM59-CKO mice. (B) Representative H&E staining images and IHC detection of Ki67, MMP-9, and Madcam1 in B16-F10 allografts (original magnification × 200). (C) Detection of MMP-9 and Madcam1 mRNA expression in B16-F10 allografts by qRT-PCR. Data are represented as mean ± SD. *p<0.05, **p<0.01, compared to tumors generated by shRNA control B16 cells in TRIM59-CKO mice.