Research Paper Volume 11, Issue 19 pp 8664—8680

Decreased RIPK1 expression in chondrocytes alleviates osteoarthritis via the TRIF/MyD88-RIPK1-TRAF2 negative feedback loop

Figure 5. TRAF2 regulates RIPK1-mediated inflammation. (A, B) Western blots and quantitative data of TRAF2 in chondrocytes transfected with Ad-shRIPK and Ad-RIPK1 in the presence and absence of IL-1β. © TRAF2 expression determined by immunofluorescence staining. Scale bar = 50 μm. (D, E) Western blots and quantitative data of p-RIPK1 and RIPK1. (F, G) Western blots and quantitative data of TRIF, MYD88, and MMPs. The experiments were repeated three times independently. Columns represent means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.