Research Paper Volume 11, Issue 23 pp 10814—10825

Long noncoding RNA LINC00958 accelerates the proliferation and matrix degradation of the nucleus pulposus by regulating miR-203/SMAD3

Figure 6. Ectopic expression of miR-203 decreased NP cell growth and inhibited ECM degradation. (A) Elevated expression of miR-203 inhibited NP cell proliferation, which was determined by MTT analysis. (B) The expression of PCNA was determined by using qRT-PCR analysis. (C) Elevated expression of miR-203 suppressed PCNA expression in the NP cells. (D) The expression of MMP-2 was determined by using qRT-PCR assay. (E) The expression of MMP-13 was determined by using qRT-PCR assay. (F) The expression of Col II was determined by using qRT-PCR assay. (G) The expression of aggrecan was determined by using qRT-PCR assay. *p<0.05, **p<0.01. GAPDH was used as the internal control. Data were showed as mean±SD.