Research Paper Volume 11, Issue 22 pp 10203—10219

Mitochondrial fission regulator 2 (MTFR2) promotes growth, migration, invasion and tumour progression in breast cancer cells

Figure 5. MTFR promotes the proliferation and migration and invasion of BC in a HIF1α- and HIF2α-dependent manner. (A) The relative mRNA levels of HIF1α and HIF2α in different cell lines (Student’s two one-tailed paired test * p<0.05). (B) Western blot of HIF1α and HIF2α in different cell lines. (C) The relative mRNA levels of HIF1α and HIF2α in different cell line (Student’s two one-tailed paired test * p<0.05). (D) Western blot of HIF1α and HIF2α in different cell lines. (E) CCK-8 assay of different cell lines (Student’s two one-tailed paired test * p<0.05). (F) The colony formation assay and statistical analysis of different cell lines (Student’s two one-tailed paired test * p<0.05). (G) The migration assay and invasion assay of different cell lines (Student’s two one-tailed paired test * p<0.05). (H) Western blot of EMT markers of different cell lines.