Figure 8. Anti-inflammatory effect of metformin on rabbit AF cells induced by LPS. (A) PGE2 production in the medium of rabbit AF cells tested by ELISA. (B) HMGB1 concentration in the medium of rabbit AF cells tested by ELISA. (C) Inflammatory-related marker protein expression in rabbit AF cells tested by western blot. ELISA results indicated that LPS induced the inflammation in rabbit AF cells as evidenced by PGE2 production and HMGB1 concentration found in the medium of rabbit AF cells cultured with LPS. Effect was inhibited by metformin. *p<0.05 to the control group. #p<0.05 compared to the cells treated with LPS.