Research Paper Volume 11, Issue 22 pp 10374—10384

Hypoxia-induced microRNA-10b-3p promotes esophageal squamous cell carcinoma growth and metastasis by targeting TSGA10

Figure 3. Hypoxia-induced miR-10b-3p enhances ESCC cell migration and invasion. (A and B) ECA109 and KYSE410 cells transfected with miR-10b-3p mimics or pc-DNA TSGA10 were seeded in serum-medium into Matrigel-free (A) or Matrigel-coated (B) upper chambers, while the lower chamber contained complete medium. After incubation for 20 hours under normoxic conditions, invading cells were fixed, photographed, and counted. (C and D) For hypoxia assays, ECA109 and KYSE410 cells transfected with miR-10b-3p inhibitor or sh-TSGA10 were seeded in serum-free medium into Matrigel-free (C) or Matrigel-coated (D) upper chambers, while the lower contained complete medium. After incubation for 20 hours under hypoxic condition, invading cells were fixed, photographed, and counted. *P < 0.05.