Research Paper Volume 11, Issue 22 pp 10422—10453

Large-scale analyses identify a cluster of novel long noncoding RNAs as potential competitive endogenous RNAs in progression of hepatocellular carcinoma

Figure 7. Correlation validation for the six lncRNAs with their associated miRNA and mRNAs in 158 clinical samples of HCC. RT-PCR was performed to detect the expression of the six lncRNAs associated miRNA and mRNAs in 158 clinical samples. Pearson correlation analysis for six key lncRNAs with their associated miRNA and mRNAs was performed based on RT-PCR results. Pearson correlograms of lncRNA-miRNA paris (A), lncRNA-mRNA pairs (B) and mRNA-miRNA pairs (C) in upregulated lncRNAs including GBAP1, MCM3AP-AS1 and SLC16A1-AS1. lncRNA-miRNA paris (D), lncRNA-mRNA paris (E) and mRNA-miRNA paris (F) in downregulated lncRNAs including C3P1, HNF4A-AS1 and DIO3OS by Pearson correlation analysis. The correlation coefficient R value ranges from -1 to 1, the color of which changes from blue to yellow. 158 clinical samples were diveded into early and late groups according to TMN stage (early = TNM I or II; late = III), the expression of the six lncRNAs associated miRNA and mRNAs was shown as heatmaps. (G) Expression of the upregulated lncRNA GBAP1, MCM3AP-AS1 and SLC16A1-AS1 and their ssociated miRNA and mRNAs. (H) Expression of th edownregulated lncRNA C3P1, DIO3OS, and HNF4A-AS1 and their ssociated miRNA and mRNAs. Log2(P) value was shown as color ranging from blue to red.