Research Paper Volume 11, Issue 22 pp 10454—10467

Abnormal gut microbiota composition contributes to the development of type 2 diabetes mellitus in db/db mice

Figure 4. Changes in the metabolic parameters of pseudo-germ-free mice after fecal transplantation from db/db and m/m mice. (A) Schedule of the present study. Host mice were treated with large doses of antibiotics for 14 consecutive days and were orally treated with fecal microbiota from db/db or m/m mice. Fecal samples were collected for 16S rRNA gene sequencing on day 29. (B) Blood glucose (Time: F2,12 = 16.24, P < 0.001; Group: F3,18 = 15.42, P < 0.001; Interaction: F6,36 = 11.33, P < 0.001). (C) Water intake (Time: F2,12 = 26.33, P < 0.001; Group: F3,18 = 22.17, P < 0.001; Interaction: F6,36 = 11.74, P < 0.001). (D) Food intake (Time: F2,12 = 13.73, P < 0.001; Group: F3,18 = 8.878, P < 0.001; Interaction: F6,36 = 6.519, P < 0.001). (E) Body weight (Time: F2,12 = 5.57, P < 0.05; Group: F3,18 = 5.319, P < 0.01; Interaction: F6,36 = 2.03, P > 0.05). Data are shown as mean ± SEM values (n=7). *P < 0.05, **P < 0.01, ***P<0.001. CONT, control; NS, not significant; SEM, standard error of the mean.