Research Paper Volume 11, Issue 22 pp 10626—10643

Circ_0006332 promotes growth and progression of bladder cancer by modulating MYBL2 expression via miR-143

Figure 7. Circ_0006332 promotes growth of bladder cancer xenograft in vivo. (A) In vivo experiment strategy involved subcutaneously injecting nude mice with T24 bladder cancer cells transfected with shRNA against circ_0006332 or the empty vector. (B) Xenograft tumor size at day 30 in the circ_0006332 shRNA and empty vector groups is shown. (C) Xenograft tumor volumes at day 30 in the circ_0006332 shRNA and empty vector groups are shown. (D) Western blot analysis shows significantly lower MYBL2 protein levels in the circ_0006332 shRNA group tumors compared with the empty vector group tumors. (E) Upregulated circ_0006332 sponges and decreases free miR-143 levels, which increases MYBL2 expression and leads to bladder cancer (BCa) tumorigenesis. Note: Data are represented as mean ± SD; *P < 0.05.