Research Paper Volume 11, Issue 22 pp 10664—10683

Competing endogenous network analysis identifies lncRNA Meg3 activates inflammatory damage in UVB induced murine skin lesion by sponging miR-93-5p/epiregulin axis

Figure 6. (A) The binding region between Ereg and miR-93-5p were predicted, and the sequences of wild-type Ereg (WT-Ereg) or mutant Ereg (MUT-Ereg) sequences were shown. (B) The directly binding between Ereg and miR-93-5p were confirmed by a luciferase reporter assay was performed with the luciferase reporter plasmids of WT-Meg3 or MUT-Meg3. (C) The protein expression of Ereg, MMP1 and MMP3 were determined by Western blot. Cells were transfected with miR-93-5p inhibitor or siRNA Meg3. (D) The inhibitory efficiency of miR-93-5p inhibitor and/or siRNA Meg3 for their effects on Ereg, MMP1 and MMP3 expression were determined by qRT-PCR. (E) The inhibitory efficiency of miR-93-5p inhibitor and/or siRNA Meg3 for their effects on inflammatory cytokines expression were determined by qRT-PCR. (F) The protein expression of Ereg, MMP1 and MMP3 were determined by Western blot. Cells were transfected with miR-93-5p mimic or pcDNA-Meg3 plasmid. (G) The effects of miR-93-5p mimic and/or Meg3 overexpressed plasmid on Ereg, MMP1 and MMP3 expression were determined by qRT-PCR. (H) The effects of miR-93-5p mimic and/or Meg3 overexpressed plasmid on inflammatory cytokines expression were determined by qRT-PCR. (I) The protein expression of Ereg, EGFR, pEGFR and MMP1 were determined by Western blot. Cells were transfected with miR-93-5p inhibitor and/or siRNA Meg3 after UVB irradiation. (J) The effects of miR-93-5p inhibitor and/or siRNA Meg3 on inflammatory cytokines expression were determined by qRT-PCR.