Research Paper Volume 11, Issue 23 pp 10839—10860

HIF-2α upregulation mediated by hypoxia promotes NAFLD-HCC progression by activating lipid synthesis via the PI3K-AKT-mTOR pathway

Figure 2. The hypoxic microenvironment induces lipid accumulation in HCC and steatotic HCC cells by upregulating HIF-2α. (A, B) Quantitative RT-PCR assessment of HIF-2α expression in HCC and steatotic HCC cells under hypoxic conditions. Transcription levels were normalized to those of β-actin. (C) Western blot analysis of HIF-2α expression in HCC and steatotic HCC cells under hypoxic conditions. β-Actin was used as the loading control. (D) Densitometric analyses of the band intensity ratios for HIF-2α/β-actin. (E) Oil red O staining and quantification in HCC and steatotic HCC cells with or without hypoxia treatment. (F, G) Triglyceride levels in HCC and steatotic HCC cells subjected to different durations of hypoxia.