Research Paper Volume 11, Issue 23 pp 10902—10922

The long non-coding RNA SNHG12 promotes gastric cancer by activating the phosphatidylinositol 3-kinase/AKT pathway

Figure 2. SNHG12 promotes the growth of GC cells. (A) SNHG12 levels in GES-1 and GC cell lines were detected by qRT-PCR. (B) The transfection efficiencies of si-SNHG12 in AGS and MGC-803 cells and pc-SNHG12 in BGC-823 cells were detected by qRT-PCR. (C) A CCK-8 assay was used to detect the proliferation of AGS and MGC-803 cells transfected with si-SNHG12, and of BGC-823 cells transfected with pc-SNHG12. (D) A colony formation assay was used to detect the colony formation abilities of AGS and MGC-803 cells transfected with si-SNHG12, and of BGC-823 cells transfected with pc-SNHG12. Data are shown as the mean ± standard error of the mean (SEM) from at least three experiments. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.