Research Paper Volume 11, Issue 23 pp 10923—10938

Kainic acid hyperphosphorylates tau via inflammasome activation in MAPT transgenic mice

Figure 6. KA-induced activation of inflammasome promotes the phosphorylation of tau. (A, B) The expression levels of IL-1β and the phosphorylation of tau were determined by western blots of samples from MAPT Tg mice treated with KA (10 mg/kg) and/or Bay11-7082 (1 mg/kg) together with KA (10 mg/kg). The KA group was given i.p. injection of 10 mg/kg KA. The Bay11-7082+KA group mice were additionally given i.p. injections of 1 mg/kg Bay11-7082. Both groups were assessed after 48 h. (C, D) The expression levels of IL-1β and the phosphorylation of tau were determined by western blots using cells treated with KA (10 μM) and/or Bay11-7082 (1 μM) together with KA (10 μM). The KA group was treated with 10 μM KA. The Bay11-7082+KA group was additionally treated with 1 μM Bay11-7082. Both groups were assessed after 48 h. The optical density of bands in western blots was analyzed by Image J software (*P < 0.05; ***P < 0.001 vs. the control group; #P < 0.05; ##P < 0.01 vs. the KA-only group).