Research Paper Volume 11, Issue 23 pp 11084—11110

α-Mangostin remodels visceral adipose tissue inflammation to ameliorate age-related metabolic disorders in mice

Figure 7. α-Man alleviates liver injury in old mice by inhibiting miR155 expression. (A) The levels of TC, TG, LDL-C and HDL-C in livers (n = 5). (B) The levels of ALT and AST in livers (n = 5). (C) H&E staining and Masson’s trichrome staining of liver tissues, and histopathological scores of individual livers on portal inflammation and fibrosis. (n = 5). Black scale bar = 100 μm, red scale bar = 50 μm. Black arrows indicate sites of portal inflammation. 0 = no significant change, 1 = minimal, 2 = mild, 3 = moderate, and 4 = severe pathology. (D) The levels of p-AKT and AKT were detected by Western blot (n = 5). β-Actin was used as an internal loading control. (E) The expression level of miR-155 in the serum and eWAT from mice (n = 5). Data were normalized to level of U6 snRNA. Data are expressed as means ± SD. #P < 0.05, ##P < 0.01, old mice vs. young mice, *P < 0.05, **P < 0.01, α-Man vs. old mice. Y, young mice; O, old mice; L, old mice administrated with 25 mg/kg α-Man; H, old mice administrated with 50 mg/kg α-Man. (F) The expression level of miR-155 in LPS stimulated RAW264.7 macrophages and BMDMs (n = 6). Data were normalized to level of U6 snRNA. Data are expressed as means ± SD. ##P < 0.01, LPS vs. vehicle, **P < 0.01, α-Man + LPS vs. LPS.