Figure 6. Effects of the active compounds containing the effective components of Epimedium, Astragaoside and Puerarin on the protein expression levels of the iron regulated hormone hepcidin in the cerebral cortex of AD transgenic mice using the immnohistochemical staining and RT-PCR. (A and B) immunostaining images and IOD values of hepcidin. (C) mRNA expression levels of the iron regulated hormone hepcidin AD transgenic mice. All data are expressed as the mean ± SD. Intergroup differences were compared with multivariate analysis of variance followed by the least significant difference test. (A) P < 0.05, vs. C57; (B) P < 0.05, vs. AD model; (C) P < 0.05, vs. DFO; (D) P < 0.05, vs. Active compounds; (E) P < 0.05, vs. Epimedium; (F) P < 0.05, vs. Astragaoside, P < 0.05, vs. Puerarin. (immunohistochemical staining, magnification × 200).