Research Paper Volume 11, Issue 23 pp 11314—11328

CircPTPRA acts as a tumor suppressor in bladder cancer by sponging miR-636 and upregulating KLF9

Figure 5. MiR-636 promotes proliferation in BC cell lines. (AD) Results of cell proliferation and colony formation assays in BC cell lines transfected with miR-636 mimic and miR-636 inhibitor. (E) Schematic diagram depicting the interaction of miR-636 and the 3’UTR of KLF9 mRNA. (F) Luciferase reporter assay results in BC cells co-transfected with miR-636 and KLF9 3’UTR. (G) Expression of KLF9 detected by western blot in BC cells transfected with miR-636 mimic and miR-636 inhibitor. (H, I) Results of CCK-8 and colony formation assays on BC cell lines transfected with KLF9 siRNAs. Data are presented as the mean ± SD of three experiments. *P < 0.05, **P < 0.01(Student’s t-test).