Research Paper Volume 11, Issue 23 pp 11369—11381

Posttranscriptional regulation of AKT by circular RNA angiomotin- like 1 mediates chemoresistance against paclitaxel in breast cancer cells

Figure 4. Effect of circAMOTL1 siRNA on PAX treatment. MDA-MB-231 cells transfected with negative control or circAMOTL1 siRNA (si-circAMOTL1) were treated with 1 μg/ml PAX for 24 hours. (A) Cell viability was detected after siRNA transfection upon PAX treatment. n=5. **p<0.01 compared to untreated negative control. ##p<0.01 compared to untreated si-circAMOTL1. (B) Cell apoptosis upon PAX treatment was stained with Annexin V/PI double staining followed by FACS analysis. Experiments were performed in triplicate. (C) Quantitation of double negative cells (alive cells). (D) Cell invasive ability upon PAX treatment was measured with matrigel invasion assay. Experiments were performed in triplicate. **p<0.01 compared to untreated negative control. ##p<0.01 compared to untreated si-circAMOTL1.