Research Paper Volume 11, Issue 23 pp 11391—11415

Melatonin protects blood-brain barrier integrity and permeability by inhibiting matrix metalloproteinase-9 via the NOTCH3/NF-κB pathway

Figure 5. Melatonin pretreatment ameliorates the effect of MMP-9 on the expression of VE-cadherin, occludin, claudin-5, and ZO-1 in the BBB in different treatment groups. Cells were treated as described in the Materials and methods for observation by immunofluorescence. (A1A8) Immunofluorescence staining of VE-cadherin; (B1B8) Immunofluorescence staining of occludin; (C1C8) Immunofluorescence staining of claudin-5; (D1D8) Immunofluorescence staining of ZO-1.