Research Paper Volume 11, Issue 23 pp 11520—11540

Bone morphogenetic protein 4 (BMP4) alleviates hepatic steatosis by increasing hepatic lipid turnover and inhibiting the mTORC1 signaling axis in hepatocytes

Figure 6. Exogenous BMP4 attenuates the development and progression of NAFLD by suppressing the mTORC1 signaling pathway. The liver samples prepared in Figure 4 were used for the following assays. (A) Total cell lysate prepared from the retrieved liver samples was subjected to Western blotting analysis to detect the expression of the members of mTORC1 signaling pathway and lipid metabolism. (B) The retrieved liver samples were paraffin-embedded, sectioned and subjected to IHC staining to detect the expression of the members of mTORC1 signaling pathway and lipid metabolism. Each assay condition was done in triplicate, and representative images are shown