Research Paper Volume 11, Issue 23 pp 11686—11721

Cytological and genetic consequences for the progeny of a mitotic catastrophe provoked by Topoisomerase II deficiency

Figure 4. Mitotic catastrophe in top2-5 diploids leads to progeny with a greater capacity for cell division than observed in the haploid. Isogenic homozygous top2-5 diploid cells were grown and spread at either low or high cell density on Petri dishes. In addition, G1/G0 cells were micromanipulated, arrayed and treated as described in Figure 2C. (A) Ability to re-bud after transient (6 h or 24 h) incubations at 37 °C of the high-density plates. The experimental procedure is described in Figure 2. (B) Clonogenic survival profile as determined on the low-density plates (mean ± s.e.m., n=3). The experimental procedure is described in Figure 1D. (C) Capability of the progeny to split apart and relationship with overall survivability. The experimental procedure is described in Figure 2C.