Research Paper Volume 11, Issue 24 pp 12624—12640

Long noncoding RNA NNT-AS1 enhances the malignant phenotype of bladder cancer by acting as a competing endogenous RNA on microRNA-496 thereby increasing HMGB1 expression

Figure 7. Knockdown of NNT-AS1 impairs bladder cancer cell growth in vivo. All the mice were injected with T24 cells that were transfected with either siNNT-AS1 or siNC. (A) Representative images of tumor xenografts collected from groups siNNT-AS1 and siNC. (B) The growth curves of subcutaneous tumor xenografts in groups siNNT-AS1 and siNC. *P < 0.05 vs. the siNC group. (C) The weights of tumor xenografts derived from either siNNT-AS1–transfected or siNC-transfected T24 cells were measured at 4 weeks post-inoculation. *P < 0.05 vs. group siNC. (D) NNT-AS1 expression in tumor xenografts obtained from groups siNNT-AS1 and siNC was evaluated via RT-qPCR. *P < 0.05 vs. the siNC group. (E) Western-blotting assessment of HMGB1 expression in the tumor xenografts derived from either siNNT-AS1–transfected or siNC-transfected T24 cells. *P < 0.05 vs. the siNC group. (F) MiR-496 expression in tumor xenografts obtained from both groups was evaluated via RT-qPCR. *P < 0.05 vs. the siNC group.