Research Paper Volume 12, Issue 1 pp 260—287

CircNF1-419 improves the gut microbiome structure and function in AD-like mice

Figure 3. CircRNA NF1-419 influences the gut microbiota composition and cholinergic system. (A) The circRNAs of circ_zfyve1-504, circ_zcchc11-811, circ_zfp652-1147, circ_zfp236-1257, circNF1-419 and circ_zranb1-1575 by reverse transcription polymerase chain reaction in Ganoderma lucidum extractions (LZ) (oral LZ of 50 mg/[kg·d]) and Hericium erinaceus extractions (HE) (oral HE of 50 mg/[kg·d]) treated SAMP8 mice brain samples lasting 24 weeks. (B) Over expression of circNF1-419 in brain could change the gut microbiota at phylum (a) and genus level (b) of SAMP8 mice, see also in Supplementary Figure 8. (C) Expression of the proteins AChE, AMP, CHRNA1 and CHRNB1 in the brain tissues of SAMP8 mice after infection of over-expressing circNF1-419 AAV. (D) Expression of the proteins AChE, AMP, CHRNA1 and CHRNB1 in the brain tissues of 12-month-old mice after infection of over-expressing circNF1-419 AAV. (E) Expression of the proteins AChE, AMP, CHRNA1 and CHRNB1 in the brain tissues of 2-month-old mice after infection of over-expressing circNF1-419 AAV. Data are presented as the means ± SD of more than 8 independent experiments, and more than 3 independent experiments in Western bolting. *p <0.05 and **p <0.01 vs. the model group by one-way ANOVA, followed by the Holm-Sidak test.