Research Paper Volume 12, Issue 1 pp 340—358

Downregulation of ABI2 expression by EBV-miR-BART13-3p induces epithelial-mesenchymal transition of nasopharyngeal carcinoma cells through upregulation of c-JUN/SLUG signaling

Figure 1. EBV-miR-BART13-3p promotes migration and invasion of NPC cells in vitro and causes enhanced metastasis of NPC cells in vivo. (A) Wound healing assay. Left panel: cell migration is measured by wound healing assay. CNE1 and S26 cells with BART13-3p overexpressed show higher migration capacity than negative control cells. Right panel: quantification of migration rate of CNE1 and S26 cells. Scale bar, 100μm. (B) Transwell migration assay. Left panel: up-regulated level of BART13-3p leads to increased migration of NPC cells using transwell assay without matrigel. Right panel: quantification of migration rate of NPC cells. Scale bar, 100μm. (C) Transwell invasion assay. Left panel: BART13-3p overexpression increases NPC cell invasion using transwell assay with matrigel. Right panel: quantification of invasion rate of NPC cells. Scale bar, 100μm. Error bars represent SEM. (D) S26-NC cells or S26-BART13-3p cells were inoculated under the right foot pads of the mice (n=8-10 per group). Popliteal lymph node metastasis on the same side were marked with immunohistochemical staining of pan-keratin. Scale bar, 200μm. (*P<0.05; **P<0.01; ***P<0.001).