Research Paper Volume 12, Issue 1 pp 370—386

Long noncoding RNA VPS9D1-AS1 augments the malignant phenotype of non-small cell lung cancer by sponging microRNA-532-3p and thereby enhancing HMGA2 expression

Figure 7. Recovery of HMGA2 expression reverses inhibitory actions of si-VPS9D1-AS1 on NSCLC cells. (A) Quantification of HMGA2 protein expression in H460 and A549 cells transfected with plasmid pc-HMGA2 or the empty pcDNA3.1 vector along with si-VPS9D1-AS1 by western blot. *P < 0.05 vs. the si-NC group. #P < 0.05 vs. the si-VPS9D1-AS1 + pcDNA3.1 group. (BF) Cell proliferation, colony formation, apoptosis, migration, and invasiveness parameters of H460 and A549 cells, treated as described above, determined by the CCK-8 assay, the colony formation assay, flow cytometry, and Transwell migration and invasiveness assays, respectively. *P < 0.05 vs. the si-NC group. #P < 0.05 vs. the si-VPS9D1-AS1 + pcDNA3.1 group.