Figure 1. Inhibition of LSD1 reduces the proliferation in both AR-positive and AR-negative PCa cells but inhibition of BRD4 has no effect on AR-negative PCa cells. (A) Western blot analysis of AR, LSD1 and BRD4 expression in PC3, DU145, LNCaP, LAPC4, C4-2 and 22Rv1 cells. (B) The indicated cancer cells were treated with different doses of SP-2509 or JQ1 for 72 h and cell proliferation was determined by MTT assay. (C) The indicated cancer cells were treated with 1μM JQ1 or SP-2509 alone, or in combination for different time periods and cell proliferation was determined by MTT assay. Graphic data are the means ± SD of four replicate experiments. Statistical significance are determined by ANOVA with: * indicates P < 0.05; *** indicates P < 0.001, # indicates no significance.