Research Paper Volume 12, Issue 1 pp 397—415

The combined effect of epigenetic inhibitors for LSD1 and BRD4 alters prostate cancer growth and invasion

Figure 2. Inhibition of LSD1 induces cell apoptosis and arrests cells in S phase but inhibition of BRD4 exhibits no apoptotic effect in PC3 and DU145 cells. (AB) Analysis of apoptosis in LNCaP, LAPC4, 22Rv1, C4-2, PC3 and DU145 cells after 72 h treatment with 1 μM JQ1 or SP-2509 alone, or in combination. Cell death was assessed using propidium iodide (PI) staining (A) or Apoptosis was assessed by Annexin-V and PI staining followed by FACS analysis (B). (C) Graphic representation of cell cycle distribution for LNCaP, LAPC4, 22Rv1, C4-2, PC3 and DU145 cells after 72 h treatment with 1 μM JQ1 or SP-2509 alone, or in combination. Duration of each cell cycle stage was assessed using PI staining followed by FACS analysis. (D) Colony formation for cells after 72 h treatment with 1 μM JQ1 or SP-2509 alone, or in combination. Graphic data are the means ± SD of four replicate experiments. Statistical significance are determined by ANOVA with: * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001, # indicates no significance.