Research Paper Volume 12, Issue 1 pp 397—415

The combined effect of epigenetic inhibitors for LSD1 and BRD4 alters prostate cancer growth and invasion

Figure 3. Knockdown of AR reduces LSD1 inhibition. (A) Immunoblot confirming knockdown of the AR in LNCaP and 22Rv1 cells. (B) Graphic representation of control and stable AR shRNA knockdown LNCaP and 22Rv1 cells treated with 1μM JQ1 or SP-2509 alone, or in combination for different time periods. Cell proliferation was determined by MTT assay. (C) Graphic representation of control and stable AR shRNA knockdown cells treated with increasing concentrations of SP-2509 or JQ1 alone, or in combination for 48 h; cell proliferation was determined by MTT assay. (D) LNCaP cells were treated with 1μM SP-2509 or JQ1 alone, or in combination with DHT or/and enzalutamide (MDV) for 72 h. Statistical difference are determined by ANOVA with: * indicates P < 0.05; ** indicates P < 0.01; *** indicates P < 0.001, # indicates no significance.