Figure 4. Expression of AR sensitizes AR-negative cells to LSD1 inhibition. (A) Immunoblot confirming ectopic expression of the AR in PC3 cells. (B) Graphic representation of control and stable AR-expressing PC3 treated with 1μM JQ1 or SP-2509 alone, or in combination for 72 hr. Cell proliferation was determined by MTT assay. (C) Graphic representation of control and stable AR-expressing PC3 cells treated with increasing concentrations of SP-2509 or JQ1 alone, or in combination for 48 h and cell proliferation were determined by MTT assay. Statistical difference are determined by ANOVA with: * indicates P < 0.05; # indicates no significance.