Research Paper Volume 12, Issue 1 pp 397—415

The combined effect of epigenetic inhibitors for LSD1 and BRD4 alters prostate cancer growth and invasion

Figure 6. LSD1 and BRD4 inhibition have different target genes in AR-positive and AR-negative PCa cells. (A) Cells were treated with 1 μM JQ1 or 1 μM SP-2509 alone, or in combination for 72 h. mRNA expression of selected AR target genes was measured by real-time PCR assay. Data were reported as the fold change in mean levels ±SD. (BC) Cells were treated with 1 μM JQ1 or 1 μM SP-2509 alone, or in combination for 72 h. Total protein lysates were analyzed by immunoblot using the indicated antibodies.