Research Paper Volume 12, Issue 1 pp 844—865

Surgery/Anesthesia disturbs mitochondrial fission/fusion dynamics in the brain of aged mice with postoperative delirium

Figure 4. Surgery/Anesthesia changed the expression of Drp1 and Mfn2 in the cytosolic fraction and in the mitochondrial fraction of the hippocampus in aged mice at 0, 6, 9, and 24 hours postoperatively. The expression of Drp1 and Mfn2 was estimated using western blotting analysis of fresh cytosolic and mitochondrial fractions of the hippocampus obtained from mice at 0, 6, 9, and 24 hours after Surgery/Anesthesia or control treatment. As compared to control condition in mice, Surgery/Anesthesia decreased Drp1 expression in the cytosolic fraction (A and C) and increased Drp1 expression in the mitochondrial fraction (B and D) of homogenates of hippocampal tissue. When compared to the control condition in mice, Surgery/Anesthesia significantly decreased the expression of Mfn2 in the cytosolic fraction (E and G) and increased Mfn2 level in the mitochondrial fraction (F and H) of homogenates of hippocampus tissue at 0, 6, 9, and 24 hours postoperatively. The data are plotted as the mean ± standard error of the mean for each group (n = 6). *p < 0.05 and **p < 0.01, compared to control.