Research Paper Volume 12, Issue 1 pp 844—865

Surgery/Anesthesia disturbs mitochondrial fission/fusion dynamics in the brain of aged mice with postoperative delirium

Figure 5. Surgery/Anesthesia changed the expression of Drp1 and Mfn2 in the cytosolic fraction and in the mitochondrial fraction of the prefrontal cortex tissues in aged mice at postoperative 0, 6, 9, and 24 hours. Immediately after Surgery/Anesthesia, there was no significant change in the expression of Drp1 both in the cytosolic fraction (A and C) and the mitochondrial fraction (B and D) as compared to that in the control condition. At 6, 9, and 24 hours postoperatively, Surgery/Anesthesia significantly decreased the expression of Drp1 in the cytosolic fraction (A and C) and increased Drp1 protein level in the mitochondrial fraction (B and D) from the prefrontal cortex tissues in mice when compared to the control condition. Additionally, Surgery/Anesthesia decreased the expression of Mfn2 in the cytosolic fraction (E and G) at 0, 6, 9, and 24 hours postoperatively. When compared to the control condition, Surgery/Anesthesia did not significantly change the expression of Mfn2 in the mitochondrial fraction (F and H) at 0 hour postoperatively. As compared to the control condition, Surgery/Anesthesia significantly increased Mfn2 expression in the mitochondrial fraction (F and H) from the prefrontal cortex tissue in mice at 6, 9, and 24 hours postoperatively. The data are plotted as the mean ± standard error of the mean for each group (n = 6). *p < 0.05 and **p < 0.01, compared to control.