Research Paper Volume 12, Issue 1 pp 866—883

Prognostic value of epithelial-mesenchymal transition markers in clear cell renal cell carcinoma

Figure 1. Analysis of the six EMT related genes in Oncomine database and TCGA database. (A) The Oncomine database was queried for the expression of CDH1, CDH2, SNAI1, SNAI2, VIM, and TWIST1 in the available datasets based on the following criteria: 1) “Cancer Type”; 2) “Gene: CDH1, CDH2, SNAI1, SNAI2, VIM, or TWIST1”; 3) “Data Type: mRNA”; 4) “Analysis Type: Cancer vs Normal Analysis”, and 5) Threshold Setting Condition (p<0.001, fold change >2, gene rank = top 10%). The 'red cells' represents gene overexpression and the 'blue cells' represent gene underexpression. The color intensity equals the percentile, i.e. Top 1%, 5%, or 10% significantly over- or underexpressed (see the legend below the grid). We found that CDH1 and SNAI2 was underexpressed in the kidney cancer vs normal datasets, while VIM was highly overexpressed. (BG) Differential mRNA expression of six EMT related genes in clear cell renal cell carcinoma (ccRCC) tumor samples and adjacent normal tissues from TCGA. Epithelial marker CDH1 mRNA expression was significantly lower in tumor samples compared with adjacent normal tissues (B); Most mesenchymal markers (CDH2, SNAI1, VIM, and TWIST1) mRNA expression was elevated in tumor samples compared with adjacent normal tissues (CG).