Figure 6. Bafilomycin A1 treatment blocks FMO3-induced anti-aging effects on liver. Levels of (A) IL-6 and (B) insulin in serum were measured by ELISA. Levels of (C) SOD, (D) MDA, (E) serum TG, (F) liver TG, (G) serum TC, and (H) liver TC were measured using commercial kits. (I) Lipid droplet accumulation was assessed via Oil Red O staining. (J) Western blotting was used to determine the levels of (K) FMO3, (L) β-gal, and (M) p16. Results are shown as the mean ± SD of eight animals per group. *p < 0.05 compared with the control group; #p < 0.05 compared with the FMO3 overexpression group.