Research Paper Volume 12, Issue 3 pp 2226—2245

Aucubin exerts anti-osteoporotic effects by promoting osteoblast differentiation

Figure 7. AU enhanced the osteoblast differentiation of femoral bone in Dex-ineduced osteoporotic mice. (A) AU enhanced the expression levels of osteoblast differentiation related proteins including collagen I, Osterix, OCN, OPN, BMP2, and P4HB in femoral bone tissues of Dex-ineduced osteoporotic mice. (B) AU enhanced the expression levels of P-Smad and P-Akt in femoral bone tissues of Dex-ineduced osteoporotic mice. (C) The effects of AU on the expression levels of Nrf2/HO-1 signaling related proteins in femoral bone tissues of Dex-ineduced osteoporotic mice, including P-DRP1, Nrf2, CAT, HO-1, HO-2, SOD-1, SOD-2, P-Smad and P-Akt. The quantification data of proteins were normalized by corresponding GAPDH and total proteins, respectively, expressed as mean ± S.D. (n=6) and analyzed using a one-way ANOVA. # P<0.05, ## P<0.01 and ### P<0.001 vs. CTRL mice, *P<0.05, **P<0.01 and ***P<0.001 Dex-treated mice.