Research Paper Volume 12, Issue 3 pp 2246—2260

The protein tyrosine kinase inhibitor, Genistein, delays intervertebral disc degeneration in rats by inhibiting the p38 pathway-mediated inflammatory response

Figure 3. Genistein suppresses the degeneration of NPCs in vitro. (A) mRNA expression of COL2A in NPCs treated with IL-1β and Genistein or SB203580. *p>0.05 (B) mRNA expression of aggrecan in NPCs treated with IL-1β and Genistein or SB203580. *p>0.05 (C) mRNA expression of collagen X in NPCs treated with IL-1β and Genistein or SB203580. *p>0.05 (D) Toluidine blue staining and cell immuohistochemmistry stainingof NPCs treated with IL-1β and Genistein. Red scale bar represents 25 μm (E) mRNA expression of TNF-α in NPCs treated with IL-1β and Genistein or SB203580. *p<0.05 (F) mRNA expression of IL-1β in NPCs treated with IL-1β and Genistein or SB203580. *p<0.05.