Research Paper Volume 12, Issue 3 pp 2261—2274

TYRO3 facilitates cell growth and metastasis via activation of the Wnt/β-catenin signaling pathway in human gastric cancer cells

Figure 3. The effects of TYRO3 downregulation or upregulation on gastric cancer (GC) cell migration and invasion in vitro and metastasis in vivo. (A) Representative images of TYRO3 downregulation or upregulation-induced morphological changes of GC cells. (B) The effects of TYRO3 downregulation or upregulation on the migratory capability of GC cells using the wound-healing assay. (C) The effects of TYRO3 downregulation or upregulation on the migratory and invasive capabilities of GC cells using the Transwell assay. (D) Representative hematoxylin and eosin staining images of lung metastases. Metastatic tumor nodules are indicated by black arrows. (E) Western blot analyses of the expression levels of TYRO3, E-cadherin, and N-cadherin in the lung metastatic nodules. (F) Immunohistochemical detection of the expression levels of TYRO3 and E-cadherin in the lung metastatic nodules (n = 6/group). Glyceraldehyde 3-phosphate dehydrogenase was used as a loading control. *P < 0.05.