Research Paper Volume 12, Issue 3 pp 2393—2407

Abnormal overexpression of G9a in melanoma cells promotes cancer progression via upregulation of the Notch1 signaling pathway

Figure 7. NICD inhibits UNC0642-induced apoptotic enhancement in melanoma cells. (A) the apoptosis of melanoma cells with the treatment of UNC0642 or both UNC0642 and NICD was detected by flow cytometry. (B) the column diagram showed the apoptosis cell number. (C) the apoptosis related protein levels were detected by western blot in melanoma cells with the treatment of UNC0642 or both UNC0642 and NICD. (D) the column diagram showed the related expression levels of target genes. (E) the apoptosis of melanoma cells with G9a knockdown or both G9a knockdown and NICD treatment was detected by flow cytometry. (F) the column diagram showed the apoptosis cell number. (G) the apoptosis related protein levels were detected by western blot in melanoma cells with G9a knockdown or both G9a knockdown and NICD treatment. (H) the column diagram showed the related expression levels of target genes. (I) the nude mice were inoculated with melanoma cells with the treatment of DMSO or UNC0642. (J) the tumor volume was measured. *P<0.05, **P<0.01 and *** P<0.001 vs. Con group; #P<0.05, ##P<0.01 and ###P<0.001 vs. UNC0642 group.